A fluorescence-labeling method for sequencing small RNA on polyacrylamide gel
نویسندگان
چکیده
منابع مشابه
A fluorescence-labeling method for sequencing small RNA on polyacrylamide gel.
A practical fluorescence-labeling method for sequencing small RNAs by the traditional 'direct read out' on polyacrylamide gel electrophoresis was established. The 3' terminus of RNA was oxidized into dialdehyde by sodium periodate and then labeled with fluorescein-5-thiosemicarbazide through the condensation reaction between carbazide and aldehyde. The fluorescence-labeled RNA was partially deg...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1996
ISSN: 1362-4962
DOI: 10.1093/nar/24.17.3472